TIRF microscopy

Total Internal Reflection Fluorescence (TIRF) microscopy allows the observation of a thin region of the sample, generally around 100 nanometers, near the interface between the sample and the glass layer of the specimen. This method strongly improves the signal-to-noise ratio. image.

Here is an extract from one of the modules that compose the e-Learning course “Vedere per Credere” (Seeing to Believe), Researchers Edition, by Dr. Spartaco Santi.

Quantum Dots

Resolution in Z

Dequenching

Quantum Dots

Resolution in Z

Dequenching


W8 Physical Cytometer

The physical cytometer W8 is the only automated platform capable of providing a label-free biophysical characterization of three-dimensional cell cultures such as spheroids or organoids. The technology, conceived by CellDynamics, measures the size, weight and mass density of said three-dimensional objects, coupling image tracking analysis with gravimetric theory. The instrument is capable of performing the sorting of 2 subpopulations, based on the previously mentioned parameters, all this preserving sample sterility and viability. These physical parameters acquire an applicative relevance that ranges from 3D Cell Culture standardization to compactness correlated readouts, from monitoring permeation activities to the evaluation of drug effectiveness, and more.

Core-chip

Mass density

Confocal Analysis

Core-chip

Mass density

Confocal Analysis


Holotomographic Microscopy

This approach permits overcoming several limitations of the current microscopy techniques, opening new perspectives in the field of label-free applications.

Specifically, Tomocube holotomographic microscopy uses optical diffraction tomography (ODT), which allows microscopists to investigate quantitatively and non-invasively on biological cells and tissues, tush avoiding the use of fluorochromes and lasers that could induce phototoxicity phenomena within the sample. ODT reconstructs the distributions of the 3D refractive index of living cells and, in doing so, provides structural and biochemical information about the cell, including the dry mass, morphology and dynamics of the cell membrane.

Holotomographic

Optical path

3D Rendering

Holotomographic

Optical path

3D Rendering